Primer

Part:BBa_K4621099

Designed by: Yanqi Liu   Group: iGEM23_SCUT-China   (2023-10-04)


Used to amplify EctOperon fragments

Paired with Ect-R, EctOperon can be amplified by PCR from the genome of strain SCUT-3 discovered in our laboratory,inserted into the pSET152PZ plasmid and connected to the promoter P24880. ectABCD-F and Ect-R primers amplified a fragment length of 3508 bp containing EctOperon.

ectabcd.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None